HIGH-COMPLEXITY HOSPITAL
"PTE. JUAN DOMINGO PERÓN"
The High Complexity Hospital "President Juan Domingo Perón" aspires to be an institution of regional, national and international importance in the field of healthcare, teaching and research. We have intelligent technology managed by a group of professional specialists that improves diagnostic quality, reduces the risks of mistakes, maximizes curative effects, reduces costs and optimizes the use of resources.
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Molecular Biology Laboratory

The Molecular Biology Laboratory began operations with the same start of operation of the High Complexity Hospital "President  Juan D. Peron" of Formosa in 2003, functioning in first instance within the structure of the clinical analysis laboratory.

Originally, it was born as a laboratory of virology where molecular biology techniques are used, in addition to the classic serological methods, for the diagnosis of pathologies of viral etiology. In this way it is constituted as a provincial reference laboratory for several viral diseases of the National Network of Laboratories which belongs to the National Laboratories and Health Institutes Administration (ANLIS).

At the beginning of 2006, the laboratory moved temporarily to an independent physical space. Tthe INCUCAI accreditation for the Histocompatibility area was obtained in 2007, responding to the requirements of the HAC and regarding solid organ transplantation.

In 2012, the laboratory began operations in its own space, a place designed and constructed in order to meet the current and future needs of the HAC, meeting the requirements of a service of these characteristics, which has ten specific work sectors, a Waiting room for patients, an extraction room, two offices, a freezers room and a machine room with its own generator. In addition, human resource was incorporated, which was formed under a continuous training system, in the area of knowledge corresponding to the requirements and new benefits were added. Thus, nucleic acid screening was incorporated into blood bank samples. New laboratory networks were added, technology was transferred in the existing networks and a cytogenetics laboratory was recently put into operation.

Currently, there are four areas work in the laboratory, each of them in charge of a specially trained professional: Virology, Histocompatibility, Autoimmunity, Genetics. Within this latter, Cytogenetic studies are in the process of being implemented.

OBJECTIVES

Provide a quality service and in accordance with the current needs in each of the areas of interference, consolidating itself as a relevant tool in the diagnosis and follow-up of pathologies, using the continuous training of human resources and transfer of knowledge and technology as an instrument for achieving that objective, valuing the human quality and respect in patient care.

 

SERVICE BENEFITS

 VIROLOGY AREA

Virological diagnosis using different techniques such as the detection of viral genomes by PCR, detection of viral antigens and antibodies against different viruses by Immunofluorescence techniques (IF) or enzyme-linked immunosorbent assay (ELISA) in different types of samples according to the clinical suspicion.

In this area, the Nucleic Acid testing (NAT) fot HIV / HBV / HCV is also performed by molecular biology. This determination is made in a specific sub-area, to samples that come from the Regional Center of provincial Hemotherapy that operates in the HAC, with state-of-the-art automated equipment pioneer in the NEA / NOA region, within the framework of the provincial blood safety programme.

 The area functions as a provincial reference for the National Networks of laboratories listed below, and as such participates in the external quality controls proposed by them:

• National network for influenza and respiratory virus

• Laboratory network for exanthematous diseases

• National surveillance network for viral gastroenteritis

• National network for dengue, yellow fever and arboviral encephalitis

• National network for hantavirus

• National network in human papillomavirus

• National network of viral infection in pregnant women and newborns

• National programme for viral hepatitis control

• HIV / AIDS laboratory network (paediatrics)

 

AUTOIMMUNITI AREA

It investigates the presence of autoantibodies associated with pathologies of autoimmune origin such as Systemic Lupus Erythematosus, Rheumatoid Arthritis, Sjogren's Syndrome, Antiphospholipid Syndrome, Vasculitis and celiac disease by Immunofluorescence techniques (IF) or Enzyme-linked immunosorbent assay (ELISA).

 

HISTOCOMPATIBILITY AREA

There are studies of compatibility between donors and recipients of solid organ transplants mainly  Kidney transplants, and hematopoietic stem cell  transplantation (bone marrow).

On the one hand, the HLA Class I (A, B) and  II (DRB, DQ) are studied by molecular biological methods (PCR - SSP). On the other hand, presence of antibodies in the receptors against the HLA of the donors is evaluated by complement-dependent cytotoxicity (CDC) technique.

The area is qualified under INCUCAI regulations and participates in the quality controls proposed by that entity.

 

GENETICS AREA

Congenital abnormalities caused by genetic factors are classified according to the magnitude of changes in genetic information in  chromosomal and genetic disorders.

Chromosome abnormalities occur as either alterations in the number of chromosomes or structural alteration .

Genetic disorders are those caused by  gene alteration (mutation).

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